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Primary Antibodies  

Rabbit anti-CERT/GPBP Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (8)
Target:

CERT/GPBP

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

CERT (GPBP) is a kinase that phosphorylates the alpha 3 chain of type IV collagen, also known as the Goodpasture antigen, on serine and threonine residues. One isoform of CERT has been shown to mediate intracellular trafficking of ceramides from the endoplasmic reticulum to the Golgi apparatus in a non-vesicular manner.

10087

COL4A3BP

collagen type IV alpha 3 binding protein

Q9Y5P4

Collagen type IV alpha-3-binding protein

Alternate Names

ceramide transfer protein; ceramide transporter; CERT; CERTL; collagen type IV alpha-3-binding protein; collagen, type IV, alpha 3 (Goodpasture antigen) binding protein; Goodpasture antigen-binding protein; GPBP; hCERT; lipid-transfer protein CERTL; MRD34; STARD11; StAR-related lipid transfer (START) domain containing 11; StAR-related lipid transfer protein 11; START domain-containing protein 11

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.