HdmX/MDM4 Antibody

Description

Rabbit anti-HdmX/MDM4 Antibody, Affinity Purified

Catalog No.A300-287A

Price$314.00 Request Bulk Quote

Data Sheet

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Secondary Antibodies Positive Controls

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Product Information
Background
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Ordering Information

Quantity:

0.1 ml (100 - 250 blots)

Reactivity:

Human

100% Sequence Identity:

Orangutan, Rhesus Monkey, Gorilla, Northern white-cheeked... more...

Format:

Whole IgG

Applications:

WB, IP, IHC-P, IF, PLA

GeneID URL:

4194

ProteinID URL:

NP_002384.2

Alternative Names:

p53-binding protein mdm4 antibody, mdm2-like p53-binding protein antibody, double minute 4 protein antibody, MDMX antibody, MRP1 antibody, MGC132766 antibody, DKFZp781B1423 antibody

Host:

Rabbit

Antibody Type:

Polyclonal

Purity:

Antigen Affinity Purified

Conjugate:

Unconjugated

Physical State:

Liquid

HdmX/MDM4 is a RING-finger domain containing protein that interacts with p53. HdmX/MDM4 may influence p53 stability through an interaction with the structurally similar MDM2, an ubiquitin-ligase that targets p53 for proteasomal degradation. HdmX/MDM4 may influence p53 by directly attenuating p53 transcriptional activity.

SubType	Description	Catalog No.
Positive Controls	Jurkat Whole Cell Lysate	CL300-103
PRIMARY ANTIBODIES	HdmX/MDM4 Antibody AbVantage™ Pack	A310-107A
PRIMARY ANTIBODIES	Rabbit anti-Phospho HdmX/MDM4 (S403) Antibody, Affinity Purified	A300-392A

Anderson VE et al., CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res. 2011 Jan 15;71(2):463-72. WB
PubMed: 21239475
Biderman L et al., MdmX is required for p53 interaction with and full induction of the Mdm2 promoter after cellular stress. Mol Cell Biol. 2012 Apr;32(7):1214-25. WB, ChIP
PubMed: 22290440
Chen W et al., Distinct p53, p53:LANA, and LANA complexes in Kaposi's Sarcoma--associated Herpesvirus Lymphomas. J Virol. 2010 Apr;84(8):3898-908. WB
PubMed: 20130056
de Lange J et al., High levels of Hdmx promote cell growth in a subset of uveal melanomas. Am J Cancer Res. 2012;2(5):492-507.
PubMed: 22957303
Forte E et al., MDM2-dependent inhibition of p53 is required for Epstein-Barr virus B-cell growth transformation and infected-cell survival. J Virol. 2009 Mar;83(6):2491-9. WB
PubMed: 19144715
Henze J et al., p53 modulation as a therapeutic strategy in gastrointestinal stromal tumors. PLoS One. 2012;7(5):e37776. WB
PubMed: 22662219
Huang B et al., Pharmacologic p53 activation blocks cell cycle progression but fails to induce senescence in epithelial cancer cells. Mol Cancer Res. 2009 Sep;7(9):1497-509. WB
PubMed: 19737973
Jin G et al., HDMX regulates p53 activity and confers chemoresistance to 3-bis(2-chloroethyl)-1-nitrosourea. Neuro Oncol. 2010 Sep;12(9):956-66. WB
PubMed: 20472715
Jobson AG et al., Cellular inhibition of checkpoint kinase 2 (Chk2) and potentiation of camptothecins and radiation by the novel Chk2 inhibitor PV1019 [7-nitro-1H-indole-2-carboxylic acid {4-[1-(guanidinohydrazone)-ethyl]-phenyl}-amide]. J Pharmacol Exp T WB
PubMed: 19741151
Kawai H et al., RING domain-mediated interaction is a requirement for MDM2's E3 ligase activity. Cancer Res. 2007 Jul 1;67(13):6026-30. WB
PubMed: 17616658
Kojima K et al., The novel tryptamine derivative JNJ-26854165 induces wild-type p53- and E2F1-mediated apoptosis in acute myeloid and lymphoid leukemias. Mol Cancer Ther. 2010 Sep;9(9):2545-57. WB
PubMed: 20736344
Lee JH et al., 14-3-3Gamma inhibition of MDMX-mediated p21 turnover independent of p53. J Biol Chem. 2011 Feb 18;286(7):5136-42. WB
PubMed: 21148311
Lee JH et al., Hypoxia activates tumor suppressor p53 by inducing ATR-Chk1 kinase cascade-mediated phosphorylation and consequent 14-3-3γ inactivation of MDMX protein. J Biol Chem. 2012 Jun 15;287(25):20898-903.
PubMed: 22556425
Lenos K et al., Alternate Splicing of the p53 Inhibitor HDMX Offers a Superior Prognostic Biomarker than p53 Mutation in Human Cancer. Cancer Res. 2012 Aug 15;72(16):4074-4084. IP, WB
PubMed: 22700878
Leventaki V et al., TP53 pathway analysis in paediatric Burkitt lymphoma reveals increased MDM4 expression as the only TP53 pathway abnormality detected in a subset of cases. Br J Haematol. 2012 Sep;158(6):763-71. IHC-P
PubMed: 22845047
Li D et al., Functional inactivation of endogenous MDM2 and CHIP by HSP90 causes aberrant stabilization of mutant p53 in human cancer cells. Mol Cancer Res. 2011 May;9(5):577-88. WB
PubMed: 21478269
Long J et al., Multiple distinct molecular mechanisms influence sensitivity and resistance to MDM2 inhibitors in adult acute myelogenous leukemia. Blood. 2010 Jul 8;116(1):71-80. WB
PubMed: 20404136
Lopez-Pajares V et al., Phosphorylation of MDMX mediated by Akt leads to stabilization and induces 14-3-3 binding. J Biol Chem. 2008 May 16;283(20):13707-13. WB
PubMed: 18356162
Lu Z et al., Protein phosphatase 1 inhibits p53 signaling by dephosphorylating and stabilizing Mdmx. Cell Signal. 2012 Dec 28;25(4):796-804. WB
PubMed: 23277204
Mandke P et al., MicroRNA-34a Modulates MDM4 Expression via a Target Site in the Open Reading Frame. PLoS One. 2012;7(8):e42034. WB
PubMed: 22870278
Marcar L et al., Mage-A cancer/testis antigens inhibit p53 function by blocking its interaction with chromatin. Cancer Res. 2010 Dec 15;70(24):10362-70. WB
PubMed: 21056992
Markey M et al., Full-length hdmX transcripts decrease following genotoxic stress. Oncogene. 2008 Nov 6;27(52):6657-66. WB
PubMed: 18711402
McEvoy J et al., Analysis of MDM2 and MDM4 Single Nucleotide Polymorphisms, mRNA Splicing and Protein Expression in Retinoblastoma. PLoS One. 2012;7(8):e42739. WB
PubMed: 22916154
Nie J et al., Smad ubiquitylation regulatory factor 1/2 (Smurf1/2) promotes p53 degradation by stabilizing the E3 ligase MDM2. J Biol Chem. 2010 Jul 23;285(30):22818-30. WB
PubMed: 20484049
Patton JT et al., Levels of HdmX expression dictate the sensitivity of normal and transformed cells to Nutlin-3. Cancer Res. 2006 Mar 15;66(6):3169-76. WB, IP
PubMed: 16540668
Pereg Y et al., Differential roles of ATM- and Chk2-mediated phosphorylations of Hdmx in response to DNA damage. Mol Cell Biol. 2006 Sep;26(18):6819-31. WB, ICC
PubMed: 16943424
Pishas KI et al., Nutlin-3a is a potential therapeutic for ewing sarcoma. Clin Cancer Res. 2011 Feb 1;17(3):494-504. WB
PubMed: 21098696
Smalley KS et al., An organometallic protein kinase inhibitor pharmacologically activates p53 and induces apoptosis in human melanoma cells. Cancer Res. 2007 Jan 1;67(1):209-17. WB
PubMed: 17210701
Song MS et al., The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex. EMBO J. 2008 Jul 9;27(13):1863-74. WB
PubMed: 18566590
Thompson T et al., 1,25-dihydroxyvitamin D3 enhances the apoptotic activity of MDM2 antagonist nutlin-3a in acute myeloid leukemia cells expressing wild-type p53. Mol Cancer Ther. 2010 May;9(5):1158-68. WB
PubMed: 20406950
Veerakumarasivam A et al., High-resolution array-based comparative genomic hybridization of bladder cancers identifies mouse double minute 4 (MDM4) as an amplification target exclusive of MDM2 and TP53. Clin Cancer Res. 2008 May 1;14(9):2527-34. IHC-P
PubMed: 18451213
Wade M et al., Hdmx modulates the outcome of p53 activation in human tumor cells. J Biol Chem. 2006 Nov 3;281(44):33036-44. WB
PubMed: 16905769
Wang H et al., A small-molecule inhibitor of MDMX activates p53 and induces apoptosis. Mol Cancer Ther. 2011 Jan;10(1):69-79. WB
PubMed: 21075910
Wang X et al., MdmX protein is essential for Mdm2 protein-mediated p53 polyubiquitination. J Biol Chem. 2011 Jul 8;286(27):23725-34. WB
PubMed: 21572037
Wang YV et al., Quantitative analyses reveal the importance of regulated Hdmx degradation for p53 activation. Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12365-70. WB
PubMed: 17640893
Waning DL et al., c-Abl phosphorylation of Mdm2 facilitates Mdm2-Mdmx complex formation. J Biol Chem. 2011 Jan 7;286(1):216-22. WB
PubMed: 21081495
Wong VC et al., p53 activation by Ni(II) is a HIF-1α independent response causing caspases 9/3-mediated apoptosis in human lung cells. Toxicol Appl Pharmacol. 2013 Apr 6. pii: S0041-008X(13)00130-0. doi: 10.1016/j.taap.2013.03.023. WB
PubMed: 23566959
Wynendaele J et al., An illegitimate microRNA target site within the 3' UTR of MDM4 affects ovarian cancer progression and chemosensitivity. Cancer Res. 2010 Dec 1;70(23):9641-9. WB
PubMed: 21084273

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