Detection of Human MCAF by Western Blot. Samples: Nuclear extract (20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-MCAF antibody (Cat. No. A300-169A) used at 0.2 and 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.
Detection of Human MCAF by Immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF (Cat. No. A300-169A Lot1) used at a dilution of 1:1,000 (1 Detection: DAB
P621, ATF-IP, hAM, ATF7IP, MCAF1, p621, hAM, ATF-IP, Activating transcription factor 7-interacting protein 1, ATF-interacting protein, ATF7-interacting protein, ATFa-associated modulator, MBD1-containing chromatin-associated factor 1, activa
Antigen Affinity Purified
MBD1-Containing Chromatin-Associated Factor (MCAF) associates with methyl CpG-binding protein (MBD1) that functions to recruit methyltransferases such as SETDB1 to chromatin. MCAF is required for the transcriptional repression and heterochromatin formation mediated by MBD1 and SETDB1.