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Primary Antibodies  

Rabbit anti-DHX15 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (9)
Target:

DHX15

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 750 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

DHX15 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX15 is the human ortholog of the yeast Prp43 protein. DHX15 has been demonstrated to function in ribosome biogenesis and pre-mRNA splicing.

1665

DHX15

DEAH-box helicase 15

O43143

Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Alternate Names

ATP-dependent RNA helicase #46; DBP1; DDX15; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15; DEAD/H box-15; DEAH (Asp-Glu-Ala-His) box helicase 15; DEAH (Asp-Glu-Ala-His) box polypeptide 15; DEAH box protein 15; HRH2; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; PRP43; PRPF43; PrPp43p; putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; RNA helicase 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.