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Rabbit anti-Rad21 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

Rad21

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 575 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Rad21, also called SCC1 (sister chromatid cohesion protein 1), is part of the mitotic cohesin complex that consist of SMC1, SMC3, SCC3 and SCC1. The cohesin complex is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination. Rad21 has been demonstrated to be cleaved by caspase proteins to trigger sister chromatid separation and during apoptotic signaling.

5885

RAD21

RAD21 cohesin complex component

O60216

Double-strand-break repair protein rad21 homolog

Alternate Names

CDLS4; double-strand-break repair protein rad21 homolog; hHR21; HR21; HRAD21; kleisin; MCD1; MGS; nuclear matrix protein 1; NXP1; NXP-1; protein involved in DNA double-strand break repair; RAD21 homolog; SCC1; SCC1 homolog; sister chromatid cohesion 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.