Bethyl Labs | THE LINK | July 2012 THE LINK News and updates from the polyclonal antibody specialists. July 2012 Actin Cytoskeleton Contributed by Tess Moskwik, Ph.D.
The actin cytoskeleton plays a key role in several cell functions such as cell polarization, migration, endocytosis, and intracellular transport. The actin cytoskeleton is composed of actin monomers (G-actin) that polymerize into actin filaments (F-actin) in response to extracellular stimuli and intracellular cues. Rho GTPases and phosphoinositide lipids stimulate the formation of actin structures such as lamellipodia (Rac GTPase), filopodia (Cdc42), and stress fibers (Rho) (1). The timing and localization of actin polymerization is regulated by actin polymerization machinery such as the WASP-Arp2/3 complex, which nucleates branched filament polymerization and formins, which nucleate unbranched actin filaments (2). The stability of polymerized actin is dynamically controlled by actin-binding proteins, which fine-tune the balance between actin depolymerization and actin nucleation, influence actin filament length, modulate actin filament assembly and turnover, and cross-link filaments into higher order actin structures (2). Finally, focal adhesions contain proteins, such as Cortactin, that bind actin and anchor the actin cytoskeleton to the extra cellular matrix (3). Because regulation of the actin cytoskeleton is involved in the processes of cell polarization and migration, study of the actin cytoskeleton is critical to understand aspects of human disease such as T-cell polarization and activation in response to antigen presenting cells (4) and cancer cell migration and invasion (5). READ MORE http://www.bethyl.com/content/bulletin_Actin Fluorescent phalloidin (green) marking actin filaments in HeLa cells.
Advance Your Research ELISA Kits for Human Serum Proteins Rely on our ELISA kits to consistently detect the presence of an antigen when used as recommended. Kits are optimized for serum, plasma and other biological fluid samples. Kits include: pre-coated 96-well strip plate, standard detection antibody, sample dilution buffer, HRP solution, TMB substrate, stop solution, 20X wash buffer, sealing tape and detailed instructions. Human ADAMTS13 ELISA Kit catalog #E88-500 http://www.bethyl.com/product/E88-500 Human Albumin ELISA Kit catalog #E88-129 http://www.bethyl.com/product/E88-129 Human Alpha-1-Antitrypsin ELISA Kit catalog #E88-122 http://www.bethyl.com/product/E88-122 Human Beta-2 Glycoprotein 1 ELISA Kit catalog #E88-142 http://www.bethyl.com/product/E88-142 Human CRP ELISA Kit catalog #E88-125 http://www.bethyl.com/product/E88-125 Human Hemoglobin ELISA Kit catalog #E88-135 http://www.bethyl.com/product/E88-135 Human IgA ELISA Kit catalog #E88-102 http://www.bethyl.com/product/E88-102 Human IgE ELISA Kit catalog #E88-108 http://www.bethyl.com/product/E88-108 Human IgG ELISA Kit catalog #E88-104 http://www.bethyl.com/product/E88-104 Human IgM ELISA Kit catalog #E88-100 http://www.bethyl.com/product/E88-100 Human Kappa ELISA Kit catalog #E88-115 http://www.bethyl.com/product/E88-115 Human Lactoferrin ELISA Kit catalog #E88-143 http://www.bethyl.com/product/E88-143 Human Lambda ELISA Kit catalog #E88-116 http://www.bethyl.com/product/E88-116 Human Transferrin ELISA Kit catalog #E88-128 http://www.bethyl.com/product/E88-128 View All ELISA Kits http://www.bethyl.com/category/other_elisa/ELISA A Body of Work Stay up-to-date with current industry information by reading this month's featured article. Interaction Proteomics Identify NEURL4 and the HECT E3 Ligase HERC2 as Novel Modulators of Centrosome Architecture. Abdallah K. Al-Hakim, Mikhail Bashkurov, Anne-Claude Gingras, Daniel Durocher and Laurence Pelletier. Mol. Cell. Proteomics, Jun 2012; 11: M111.014233 CEP97 - http://www.bethyl.com/product/A301-345A CP110 - http://www.bethyl.com/product/A301-344A HERC2 - http://www.bethyl.com/product/A301-905A VIEW ABSTRACT http://www.ncbi.nlm.nih.gov/pubmed/22261722
A Beacon of Trust Our new Bethyl Laboratories Seal certifies that our antibodies are 100% manufactured and qualified to the highest standards at our sole facility in Texas. Free Box of 10 ReliaBLOT® Western Blot Gels when you buy TWO Polyclonal Antibodies.* Use Promo Code 2ABS+GEL when checking out. View ReliaBLOT® Gels http://www.bethyl.com/category/pca_accessory_reliablot *Offer valid through July 31, 2012. May not be combined with any other offers. U.S. customers only. Offer applies to the following products: any Polyclonal Antibody (Cat. No. A300-XXXA – A303-XXXA) and ReliaBLOT® Western Blot Gels of various sizes and percentages (WB101-XXXXXG – WB102-XXXXXG). New Products Each month, we release a series of new antibodies. For a complete listing of products, click on the button below. NEW Antibodies http://www.bethyl.com Bethyl Laboratories | Seeing Beyond. We are committed to providing you timely information and always welcome your feedback. If there is a topic of interest you have in mind, please email us at info@bethyl.com. Some of our products include: Polyclonal and Monoclonal antibodies - http://www.bethyl.com Secondary antibodies and conjugates - http://www.bethyl.com/search/search_2nd Duolink In Situ PLA products and pre-qualified antibodies - http://www.bethyl.com/category/other_duolink/ ELISA kits - http://www.bethyl.com/category/other_elisa/ELISA Custom Antibody Service - http://www.bethyl.com/content/customservices ReliaBLOT® western blot gels, buffers and reagents - http://www.bethyl.com/category/pca_accessory_reliablot/WesternBlot P.O. Box 850 | Montgomery, Texas 77356 | 800.338.9579 | bethyl.com
 
Actin Cytoskeleton #E88-500 #E88-129 #E88-122 #E88-142 #E88-125 #E88-135 #E88-102 #E88-108 #E88-104 #E88-100 #E88-115 #E88-143 #E88-116 #E88-128 View All CEP97 CP110 HERC2 View Abstract View ReliaBLOT Gels NEW Antibodies Polyclonal and Monoclonal antibodies Secondary antibodies and conjugates Duolink In Situ ELISA kits Custom Antibody Service ReliaBLOT Bethly Twitter Facebook Email