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  • Cell Lysate Preparation - NETN Method
    Protocol
    This protocol outlines cell lysate preparation using the NETN method. This includes the recipes for reagents and the procedures for adherent cells and suspension culture.
  • Cell Lysate Preparation - Nuclear Extract
    Protocol
    Extracts prepared from the isolated nuclei of cultured cells can be used in functional studies and for the purification of proteins. This protocol adapted from Mirmira Lab, University of Virginia outlines the steps and processes required.
  • Cell Lysate Preparation - RIPA Method
    Protocol
    RIPA (Radioimmunoprecipitation Assay) - Buffer is a reagent used in cell lysis experimentation, to enable rapid, efficient solubilization of proteins. By minimizing non-specific protein binding, specific binding interactions can be easily studied and are commonly used in immunoprecipitation experiments.
  • Immunoprecipitation with Agarose-Immobilized Antibody
    Protocol
    See the protocol for immunoprecipitation with agarose-immobilized antibody, including the reagents needed and procedure outline.
  • Immunoprecipitation Protocol
    Protocol
    This protocol outlines the reagents, buffer and step-by-step procedure to be used with immunoprecipitation experimentation. To be used with immunoblotting and western blotting research methods
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