ANTIBODIES TO SWI/SNF-LIKE CHROMATIN-REMODELING COMPLEXES
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of
chromatin), and BAFs (BRG1-associated factors), have been identified as
components of the mammalian SWI/SNF-like chromatin-remodeling protein
complexes. These multi-protein complexes are proposed to function as ATP-driven
motors that translocate along DNA and destabilize nucleosomal structures to
facilitate transcription factor binding. Two fractions of SWI/SNF-like
complexes, SWI/SNF-A (BAF) and SWI/SNF-B (PBAF), have been isolated. The
SWI/SNF-A (BAF) complex is defi ned by the presence of a BAF250/ARID1A subunit
and either a SMARCA/BRG1 or SMARCA2/BRM subunit, while the SWI/SNF-B (PBAF)
complex lacks the BAF250/ARID1A subunit and contains a SMARCA4/Brg1 subunit and
a polybromo/BAF180 subunit. In addition to these defining subunits, the
SWI/SNF-A (BAF) and SWI/SNF-B (PBAF) complexes possess multiple subunits of
other SMARCs and/or BAFs that are present in various combinations to provide
the mammalian cell with a functional variety of SWI/SNF-like
chromatin-remodeling complexes. This growing family of interacting proteins has
also been shown to associate with the N-CoR (nuclear receptor co-repressor)
deacetylase complex and various nuclear transcription factors. Current
information indicates an important role for these complexes in the regulation
of cell growth and proliferation. Future studies will likely demonstrate that
the SWI/SNF proteins are important to many aspects of development and
tumorigenesis.
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Detection of Human and Mouse BAF57/SMARCE1 by
WB (human & mouse) and Immunoprecipitation (human).
Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg
for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.
Antibody: Affinity purified rabbit anti-BAF57/SMARCE1
antibody (Cat. No. A300-810A)
used for Western Blot at 0.1 mcg/ml (A) and at 1 mcg/ml (B) and used for
Immunoprecipitation at 3 mcg/mg lysate (B). For blotting immunoprecipitated
BAF57/SMARCE1, ReliaBLOT® Reagents and Procedures (Cat. No.
WB120) were used. Detection: Chemiluminescence with
exposure times of 3 minutes (A and B). |
Detection of Human SMARCC1/BAF155 by Western
Blot and Immunoprecipitation.
Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for
IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity
purified rabbit anti-SMARCC1/BAF155 antibody (Cat. No.
A301-020A) used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP
at 3 mcg/mg lysate. SMARCC1/BAF155 was also immunoprecipitated by rabbit
anti-SMARCC1/BAF155 antibodies (Cat. Nos.
A301-019A and A301-021A),
which recognize other epitopes. For blotting immunoprecipitated SMARCC1/BAF155,
ReliaBLOT® Reagents and Procedures (Cat. No.
WB120) were used. Detection: Chemiluminescence with
exposure times of 30 seconds (A) and 10 seconds (B). |
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